2.5. Import expression log2FCs in normal vs. tumor¶
You will import gene expression log2FCs between normal vs. tumor condition in all TCGA GBM patients. and map them to the colors of nodes.
- Select the Network tab of previously created network from
Cluster 3
in the original network.
- Select .
In the Import Attributes dialog,
- Be sure Attributes for:
Node
to be checked. - Choose a CSV file to import:
tcga_gbm_exp_log2fc.csv
- Check The first line in a CSV file contains column names
- Key Column in Annotation File:
Gene_Symbol
- Key Attribute in Network:
hgnc_symbol
- Click the OK button.
- Be sure Attributes for:
Now you can see a newly added column
log2FC
in the node table as shown in below:
In the Visual Mapping (Nodes) window,
- Click icon.
- Choose
log2FC
in the drop-down list. - Click to show up a palette chooser.
- Select (Red-Yellow-Blue).
- Click to map
Red
to the maximum andBlue
to the minimum value. - Check to show a list of node names with their mapped colors ranked by values.
- Click the Apply button.
The final network of
Cluster 3
is shown below:
- Repeat above steps on the network of
Cluster 5
.
Tip
How to add more gradation points and colors.
- You can add any number of gradation points by clicking desired positions on the color bar.
- Once you have a gradation point, you can edit its color by double-clicking the point.
- You can also move the points by dragging them.